MCF7 cells were subjected to SDS PAGE followed by western blot with FNab02832(ERCC1 antibody) at dilution of 1:300
IP Result of anti-ERCC1 (IP:FNab02832, 3ug; Detection:FNab02832 1:300) with MCF-7 cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human testis using FNab02832(ERCC1 antibody) at dilution of 1:50
Background
Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links(ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair(HDR) of DNA double-strand breaks, in conjunction with SLX4.